علوم زیستی دریا
Razieh Nasrolahzadeh; Alireza Safahieh; Hossein Zolgharnein; Issac Zamani; Kamal Ghanemi
Abstract
Bisphenol-A (BPA) is one of the most important endocrine disrupters that has arrived to the environment, because of this reason, it has been developed into a detrimental material for human beings and other organisams. There are several ways for degradation or removal of industrial pollutants, ...
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Bisphenol-A (BPA) is one of the most important endocrine disrupters that has arrived to the environment, because of this reason, it has been developed into a detrimental material for human beings and other organisams. There are several ways for degradation or removal of industrial pollutants, in which the bacterial communities plays a major role in the biodegradation of BPA in the environment.In this study, contaminated sediments were collected in Mousa Creek. The isolates were identified by biochemical tests and 16S rRNA gene sequence analysis. The isolated Bacteri was Pseudomonas putida. Growth and degradation ability of isolated baccteri was measured in 100, 200 and 300 ppm of BPA in 24-hour intervals for 6 days. Bacterial growth rate was detected with spectrophotometer at 600 nm. Efficiency of BPA degradation was performed by Liquid-liquid extraction method and measured by HPLC. This bacteri was able to grow at concentrations of 100, 200 and 300 ppm of BPA. So, the biodegradation results from mineral salt medium (MSM) indicated that Pseudomonas putida have the best degradation efficiency at the 200 ppm of the BPA. Biodegredation result by HPLC method show that the percentage of the degradation efficiency at the 200 ppm was 82.8 percent.These results demonstrated this indigenous bacteria (Pseudomonas putida) can be used to improve the bacterial communities contaminated by Bisphenol A and to eliminate the pollutants from the costal environment.
علوم زیستی دریا
maryam moazami; Mohammad Ali Salari-Aliabadi; bita archangi; Hossein Zolgharnein; Ahmad ghasemi
Abstract
In this study, genetic diversity of Pinctada persica population was evaluated using PCR-RFLP method. To determine and compare genetic diversity of P. persica populations in khark, shidvar, hendurabi and larak islands, 51 samples were collected from 1-6 m depths. DNA of samples was extracted by CTAB method. ...
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In this study, genetic diversity of Pinctada persica population was evaluated using PCR-RFLP method. To determine and compare genetic diversity of P. persica populations in khark, shidvar, hendurabi and larak islands, 51 samples were collected from 1-6 m depths. DNA of samples was extracted by CTAB method. A pair of primers of Pinctada persica was used for polymerase chain reaction. Enzymatic digestion of PCR yield was performed using, AvaΙΙ, DdeΙ, HindIII, SfaNΙ and TaqΙ restriction enzymes. DNAs were collected and stained with silver staining after subjected to poly acrylamide gel electrophoresis The results revealed that electrophoresis and enzymatic digestion fragments of the 51 samples collected from four stations in the northern coast of Persian Gulf with all 5 enzymes were the same The results of this study confirm that Pinctada persica Based on the studied gene is completely homogenous in these stations and show no genetic diversity.
علوم زیستی دریا
Halimeh Rajabi; Hossein Zolgharnen; Ahmad Savari; Alireza Amouzandeh; Nabi Jomehzadeh
Abstract
In this paper, the venom apparatus of the marine cone snail C. frigidus that is peptide toxins manufacture section with pharmacological effect have been studied.The venom apparatus of about 6 specimens were removed and divided to 3 parts: The venom bulb, the proximal portions of the venom apparatus (close ...
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In this paper, the venom apparatus of the marine cone snail C. frigidus that is peptide toxins manufacture section with pharmacological effect have been studied.The venom apparatus of about 6 specimens were removed and divided to 3 parts: The venom bulb, the proximal portions of the venom apparatus (close to venom bulb), the distal portions of the venom apparatus (close to pharynx). Then were fixed in Bouin's for 48 hours and transferred to 70% ethanol. After dehydration and Paraffin embedded, they were cut by microtome, collected on glass slides, stained with Haematoxylin-Eosin and studied by light microscope. The results showed that the venom bulb has longitudinal, transversal muscles, and a row of cubic cells with round nuclei. The proximal and the distal parts are composed of elongated cylindrical cells with the basal ganglia. The epithelial cells of the venom apparatus have abundant granules and in the distal part that are larger than the proximal. The passageway between the lumen of the venom duct and the pharynx is a channel that lead to the radula bag containing radula with different degrees of a formation. So, C. frigidus venom production and delivery was performed by synthesis in the venom apparatus, storage toxin in the radula teeth and the venom injection is done by the venom bulb and the best section for toxin peptide extraction is the distal portion of the venom apparatus. The reason is that this part, is active in the secretion of mature toxins that can use for Medicinal purposes.
علوم زیستی دریا
hedayat yavarmoghadam; h zolgharnein; Mohammad Ali Salari Aliabadi; s keyvan; Mohamad Modarresi
Abstract
In this present study genetic diversity of cuttlefish (Sepia pharaonis) populations were investigated using microsatellite markers. Total 51 samples were collected from Bandarabass and Bushehr regions. Tissue sample of arm tips (tentacle) were preserved in 96% ethanol alcohol until using in biotechnology ...
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In this present study genetic diversity of cuttlefish (Sepia pharaonis) populations were investigated using microsatellite markers. Total 51 samples were collected from Bandarabass and Bushehr regions. Tissue sample of arm tips (tentacle) were preserved in 96% ethanol alcohol until using in biotechnology laboratory of Khorramshahr University of Marine Science and Technology. Genomic DNA was extracted with CTAB method. The quality and quantity of extracted DNA was assessed by 1% agarose gel electrophoresis and spectrophotometry, respectively. Polymerase chain reaction conducted with 6 pairs of microsatellite primers. PCR products were electrophoresised on 8% polyacrylamide gel and stained with silver nitrate. These primers were shown 4 pairs of polymorph and 2 pairs of monomorp. Allele Sizes were measured in populations then genetic parameter were calculated using Arlequin and Gen Alex Programs and phylogenetic relationship was determinated and drawn using TFPGA Program. Result obtained showed genetic distance and resemblance distance is 0.282 and 0.754, respectively and genetic differentiation was present 0.031. The findings of present study showed low levels of genetic differentiation but significant between of populations and S. pharaonis were likely two different population. These findings provide useful information for conservation and management of this species in the Persian Gulf.
علوم زیستی دریا
Sima Sarmadian; Alireza Safahieh; Hossein Zolgharnein; Bita Archangi; Mahmood Hashemi Tabar
Abstract
Heavy metals in high concentrations in the environment, is caused serious damage in metabolic, physiologic and structural organisms. Cadmium as the second most toxic metal in marine environments is considered. Bivalvia especially oysters are Suitable bioindicators due to its high filtration rates, immobility ...
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Heavy metals in high concentrations in the environment, is caused serious damage in metabolic, physiologic and structural organisms. Cadmium as the second most toxic metal in marine environments is considered. Bivalvia especially oysters are Suitable bioindicators due to its high filtration rates, immobility and lack of regulatory systems for removing of heavy metals. Crassostrea sp. is new species has wide distribution in Bandar Emam Khomeini. This species is the best for using toxicity testing to determine the effects of heavy metal pollutants in the environment. The aim this study is determination of Medium Lethal Concentration (LC5096h), Maximum Allowable Toxicant Concentration (MATC) and Lowest Observed Effect Concentration (LOEC) of Cadmium in Crassostrea sp. oyster. Fifty oysters (5.3±0.76 gr) were collected from Bandar Emam Khomaini and transported to laboratory. After Acclimatization (for 7 days), the 96-h LC50 tests were conducted (static Method) according to standard instruction O.E.C.D. The 96 h LC50, NOEC and LOEC were 15.8, 1.58 and 2.9 mg/l respectively. The LC50 correlation whit in 24 h and 96 h were showed Linear equation y=-0.4225x+54.35.
Ahmad Shadi; Hosein Zolgharnein; MohamadAli Salari-aliabadi; Mohamad Bagher Nabavi; Mohamad Taghi Ronagh
Abstract
Using 5 microsatellite loci Sillago sp. From Hormozgan North Persian Gulf were investigated through 68 specimens in two stations: Minab(31 specimens) and Bandar Lengeh(38 specimens). DNA was extracted using modified CTAB(Hexadecyl trimethyl-ammonium bromide) protocol. 5-17 alleles were observed in 5 ...
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Using 5 microsatellite loci Sillago sp. From Hormozgan North Persian Gulf were investigated through 68 specimens in two stations: Minab(31 specimens) and Bandar Lengeh(38 specimens). DNA was extracted using modified CTAB(Hexadecyl trimethyl-ammonium bromide) protocol. 5-17 alleles were observed in 5 loci with 8.4 mean allelic frequency. Observed and expected heterozygosity values was calculated (0.115<Ho<0.792) ,(0.598<He<0.902) . Genetic distance and identity according to Nei was estimated 0.520 and 0.595 respectively which shows genetic distance of genus level. Significant deviation from Hardy-Weinberg equilibrium (p<0.001) was observed at all loci at two stations. Analysis of Molecular Variance(AMOVA) of Genetic differentiation index(Fst) revealed mean significant (p=0.001) differentiation between two studied regions and 3.90 gen flow. Despite low distance of two studied regions (180km) there are probably two distinct populations of Sillago sp. which should be considered in management efforts.